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Azospirillum palustre sp. nov., a methylotrophic nitrogen-fixing species isolated from raised bog.
Tikhonova, Ekaterina N; Grouzdev, Denis S; Kravchenko, Irina K.
Affiliation
  • Tikhonova EN; Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
  • Grouzdev DS; Institute of Bioengineering, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
  • Kravchenko IK; Winogradsky Institute of Microbiology, Federal Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.
Int J Syst Evol Microbiol ; 69(9): 2787-2793, 2019 Sep.
Article in En | MEDLINE | ID: mdl-31237535
Nitrogen-fixing bacterial strain, designated B2T, was isolated from methane-oxidation enrichment originating from a Sphagnum-dominated raised peatland in Tver region, Russia, and its phenotypic, chemotaxonomic and genomic characteristics were investigated. Cells of isolate were Gram-negative, aerobic, rod or spiral-shaped, with motility provided by a single polar flagellum in liquid media and peritrichous flagella on solid media. Strain was able to grow at 15-40 °C, pH 5.5-8.5 and tolerated NaCl to 2.0 % (w/v). Strain B2T gave positive amplification for dinitrogen reductase (nifH gene) and acetylene reduction activity was recorded up to 1250 nmol ethylene h-1 (mg protein)-1. Analysis of 16S rRNA showed that B2T represents a member of the genus Azospirillum and had the highest sequence similarity with A. humicireducens SgZ-5T (97.92 %). The predominant quinone system was ubiquinone Q-10 and the major fatty acids were C18 : 1ω7, C16 : 1ω7 and C16 : 0. The strain was facultative methylotrophic and used methanol and formate for the growth. Genome sequencing revealed a genome size of 8.0 Mbp and a G+C content of 67.8 mol%. The mxaFI genes encoding methanol dehydrogenase were absent, but a homologous xoxF gene was detected. The genes encoding enzymes involved in the biosynthesis of tetrahydromethanopterin (H4MPT) (formaldehyde oxidation) and NAD-linked formate dehydrogenase (fdsABG) were identified. Pairwise determined whole genome average nucleotide identity (gANI) values confirmed that strain B2T represents a novel species, for which we propose the name Azospirillum palustre sp. nov. with the type strain B2T (VKM B-3233T, КСТС 62613Т).
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phylogeny / Azospirillum / Wetlands / Nitrogen Fixation Type of study: Prognostic_studies Country/Region as subject: Asia / Europa Language: En Journal: Int J Syst Evol Microbiol Journal subject: MICROBIOLOGIA Year: 2019 Document type: Article Affiliation country: Russia Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phylogeny / Azospirillum / Wetlands / Nitrogen Fixation Type of study: Prognostic_studies Country/Region as subject: Asia / Europa Language: En Journal: Int J Syst Evol Microbiol Journal subject: MICROBIOLOGIA Year: 2019 Document type: Article Affiliation country: Russia Country of publication: United kingdom