Macrocystis pyrifera source of nutrients for the production of carotenoids by a marine yeast Rhodotorula mucilaginosa.
J Appl Microbiol
; 127(4): 1069-1079, 2019 Oct.
Article
in En
| MEDLINE
| ID: mdl-31237965
ABSTRACT
AIMS:
To evaluate an aqueous extract of Macrocystis pyrifera as a nutrient source for the production of carotenoids by a marine Rhodotorula mucilaginosa isolated from seaweed samples. MATERIALS ANDRESULTS:
The effect of different culture conditions on the concentration of biomass and total pigments was evaluated using a Box-Behnken experimental design. The seaweed extract contained 15% w w-1 of protein and 20% w w-1 of carbohydrate; the main sugar in this fraction was trehalose (78%). The culture conditions that maximize the total pigment concentration (1·84 ± 0·03 mg l-1 ) were initial pH equal to 7, yeast extract as nitrogen source at a concentration of 4 g l-1 , seaweed extract concentration at 25% v v-1 , incubation performed at 25°C and 150 rev min-1 during 6 days. Under optimal growth conditions, three carotenoids were identified among the pigments produced by R. mucilaginosa, lycopene (38·4 ± 9·4%), ß-carotene (21·8 ± 1·5%) and astaxanthin (1·8 ± 0·3%).CONCLUSIONS:
Carotenoids of commercial interest (lycopene, ß-carotene and astaxanthin) can be produced using a marine R. mucilaginosa cultivated with an aqueous extract of M. pyrifera as nutrient source. The total pigment concentration in the culture ranged between 0·82 and 1·84 mg l-1 , and was significantly affected by the concentration of the seaweed extract, and yeast extract. SIGNIFICANCE AND IMPACT OF THE STUDY This work demonstrates that M. pyrifera can be used as a nutrient source for the production of carotenoids by the marine yeast.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rhodotorula
/
Carotenoids
/
Macrocystis
Language:
En
Journal:
J Appl Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
Chile