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ONRAB® oral rabies vaccine is shed from, but does not persist in, captive mammals.
Sobey, Kirk G; Jamieson, Sarah E; Walpole, Aaron A; Rosatte, Rick C; Donovan, Dennis; Fehlner-Gardiner, Christine; Nadin-Davis, Susan A; Davies, J Chris; Kyle, Christopher J.
Affiliation
  • Sobey KG; Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Trent University, Peterborough, Ontario K9L 0G2, Canada.
  • Jamieson SE; Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Trent University, Peterborough, Ontario K9L 0G2, Canada. Electronic address: sarah.jamieson@ontario.ca.
  • Walpole AA; Wildlife Section, Ontario Ministry of Natural Resources and Forestry, 300 Water Street, Peterborough, Ontario K9J 8M5, Canada. Electronic address: aaron.walpole@ontario.ca.
  • Rosatte RC; Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Trent University, Peterborough, Ontario K9L 0G2, Canada. Electronic address: rcr99@nexicom.net.
  • Donovan D; Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Trent University, Peterborough, Ontario K9L 0G2, Canada. Electronic address: ddonovan2421@gmail.com.
  • Fehlner-Gardiner C; Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, PO Box 11300, Station H, Nepean, Ontario K2H 8P9, Canada. Electronic address: christine.fehlner-gardiner@canada.ca.
  • Nadin-Davis SA; Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, PO Box 11300, Station H, Nepean, Ontario K2H 8P9, Canada. Electronic address: susan.nadin-davis@canada.ca.
  • Davies JC; Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Trent University, Peterborough, Ontario K9L 0G2, Canada. Electronic address: jchris.davies@bell.net.
  • Kyle CJ; Natural Resources DNA Profiling and Forensics Centre, 2140 East Bank Drive, DNA Building, Trent University, Peterborough, Ontario K9J 7B8, Canada. Electronic address: christopherkyle@trentu.ca.
Vaccine ; 37(31): 4310-4317, 2019 07 18.
Article in En | MEDLINE | ID: mdl-31248686
ABSTRACT
ONRAB® is a human adenovirus rabies glycoprotein recombinant vaccine developed to control rabies in wildlife. To support licensing and widespread use of the vaccine, safety studies are needed to assess its potential residual impact on wildlife populations. We examined the persistence of the ONRAB® vaccine virus in captive rabies vector and non-target mammals. This research complements work on important rabies vector species (raccoon, striped skunk, and red fox) but also adds to previous findings with the addition of some non-target species (Virginia opossum, Norway rats, and cotton rats) and a prolonged period of post vaccination monitoring (41 days). Animals were directly inoculated orally with the vaccine and vaccine shedding was monitored using quantitative real-time PCR applied to oral and rectal swabs. ONRAB® DNA was detected in both oral and rectal swabs from 6 h to 3 days post-inoculation in most animals, followed by a resurgence of shedding between days 17 and 34 in some species. Overall, the duration over which ONRAB® DNA was detectable was shorter for non-target mammals, and by day 41, no animal had detectable DNA in either oral or rectal swabs. All target species, as well as cotton rats and laboratory-bred Norway rats, developed robust humoral immune responses as measured by competitive ELISA, with all individuals being seropositive at day 31. Similarly, opossums showed good response (89% seropositive; 8/9), whereas only one of nine wild caught Norway rats was seropositive at day 31. These results support findings of other safety studies suggesting that ONRAB® does not persist in vector and non-target mammals exposed to the vaccine. As such, we interpret these data to reflect a low risk of adverse effects to wild populations following distribution of ONRAB® to control sylvatic rabies.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rabies / Rabies virus / Rabies Vaccines / Immunogenicity, Vaccine / Animals, Wild Limits: Animals Language: En Journal: Vaccine Year: 2019 Document type: Article Affiliation country: Canada

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rabies / Rabies virus / Rabies Vaccines / Immunogenicity, Vaccine / Animals, Wild Limits: Animals Language: En Journal: Vaccine Year: 2019 Document type: Article Affiliation country: Canada