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Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase.
Sakurai, Takayuki; Kamiyoshi, Akiko; Takei, Norio; Watanabe, Satoshi; Sato, Masahiro; Shindo, Takayuki.
Affiliation
  • Sakurai T; Department of Life Innovation, Institute for Biomedical Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan. tsakurai@shinshu-u.ac.jp.
  • Kamiyoshi A; Department of Cardiovascular Research, School of Medicine, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan. tsakurai@shinshu-u.ac.jp.
  • Takei N; Department of Life Innovation, Institute for Biomedical Sciences, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.
  • Watanabe S; Department of Cardiovascular Research, School of Medicine, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano, 390-8621, Japan.
  • Sato M; Department of Molecular Therapeutics, Center for Food and Medical Innovation, Institute for the Promotion of Business-Regional Collaboration, Hokkaido University, Kita-21 Nishi-11, Kita-ku, Sapporo, 001-0021, Japan.
  • Shindo T; Animal Genome Research Unit, Division of Animal Science, National Institute of Agrobiological Sciences, Ibaraki, 305-8602, Japan.
Sci Rep ; 9(1): 9923, 2019 07 09.
Article in En | MEDLINE | ID: mdl-31289302
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymerase Chain Reaction / Taq Polymerase / CRISPR-Cas Systems / Gene Editing / Mutation Limits: Animals Language: En Journal: Sci Rep Year: 2019 Document type: Article Affiliation country: Japan Country of publication: United kingdom
Fulltext
Add to My VHL
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymerase Chain Reaction / Taq Polymerase / CRISPR-Cas Systems / Gene Editing / Mutation Limits: Animals Language: En Journal: Sci Rep Year: 2019 Document type: Article Affiliation country: Japan Country of publication: United kingdom