Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root Initiation in Arabidopsis.
Curr Biol
; 29(15): 2443-2454.e5, 2019 08 05.
Article
in En
| MEDLINE
| ID: mdl-31327713
ABSTRACT
How plant cells re-establish differential growth to initiate organs is poorly understood. Morphogenesis of lateral roots relies on the asymmetric cell division of initially symmetric founder cells. This division is preceded by the tightly controlled asymmetric radial expansion of these cells. The cellular mechanisms that license and ensure the coordination of these events are unknown. Here, we quantitatively analyze microtubule and F-actin dynamics during lateral root initiation. Using mutants and pharmacological and tissue-specific genetic perturbations, we show that dynamic reorganization of both microtubule and F-actin networks is necessary for the asymmetric expansion of the founder cells. This cytoskeleton remodeling intertwines with auxin signaling in the pericycle and endodermis in order for founder cells to acquire a basic polarity required for initiating lateral root development. Our results reveal the conservation of cell remodeling and polarization strategies between the Arabidopsis zygote and lateral root founder cells. We propose that coordinated, auxin-driven reorganization of the cytoskeleton licenses asymmetric cell growth and divisions during embryonic and post-embryonic organogenesis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Proteins
/
Actins
/
Arabidopsis
/
Plant Roots
/
Microtubules
Language:
En
Journal:
Curr Biol
Journal subject:
BIOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
Germany