A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids.
Methods Mol Biol
; 2030: 263-275, 2019.
Article
in En
| MEDLINE
| ID: mdl-31347124
The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields.To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents such as Marfey's reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Mass Spectrometry
/
Amino Acids
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
United States
Country of publication:
United States