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Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps.
Liu, Yuchen; Yang, Yingdong; Xu, Jing; Dong, Xiaowei; Gu, Xiaobin; Xie, Yue; Lai, Weimin; Jing, Bo; Peng, Xuerong; Yang, Guangyou.
Affiliation
  • Liu Y; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: liuyuchen1229@163.com.
  • Yang Y; Panzhihua Academy of Agricultural and Forestry Sciences, Panzhihua 617000, China. Electronic address: pzhyydong@163.com.
  • Xu J; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: xujing90@hotmail.com.
  • Dong X; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: dongxiaowei1226@outlook.com.
  • Gu X; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: guxiaobin198225@126.com.
  • Xie Y; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: zhandegaokandey123@163.com.
  • Lai W; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: nwm_mm2004@163.com.
  • Jing B; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: jingbo@sicau.edu.cn.
  • Peng X; Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: pxuerong@aliyun.com.
  • Yang G; Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China. Electronic address: guangyou1963@aliyun.com.
Vet Parasitol ; 272: 58-63, 2019 Aug.
Article in En | MEDLINE | ID: mdl-31395206
ABSTRACT
Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode of Taenia multiceps (Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development of appropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B (AgB) and thioredoxin peroxidase (TPx) from T. multiceps were cloned and expressed using a prokaryotic system, molecular characterization of Tm-AgB was determined by bioinformatical analyses. The serological diagnostic potentials of rTm-AgB and rTm-TPx were evaluated by indirect ELISA and compared with those of previously reported rTm-AnxB2, rTm-HSP70, and rTm-GST. The results showed that Tm-AgB is a specific lipoprotein of cestodes with good thermal stability. The ELISA assay showed that rTm-AgB exhibited a sensitivity of 95.8% and a specificity of 87.5%, indicating its strong potential for serological diagnosis of T. multiceps.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Taenia / Taeniasis / Peroxiredoxins / Antigens, Helminth Type of study: Diagnostic_studies / Evaluation_studies Limits: Animals Language: En Journal: Vet Parasitol Year: 2019 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Taenia / Taeniasis / Peroxiredoxins / Antigens, Helminth Type of study: Diagnostic_studies / Evaluation_studies Limits: Animals Language: En Journal: Vet Parasitol Year: 2019 Document type: Article