DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea.
Methods Mol Biol
; 2046: 189-206, 2019.
Article
in En
| MEDLINE
| ID: mdl-31407306
ABSTRACT
Methylotrophic methanogenic archaea are an integral part of the carbon cycle in various anaerobic environments. Different from methylotrophic bacteria, methylotrophic methanogens assimilate both, the methyl compound and dissolved inorganic carbon. Here, we present DNA- and RNA-stable isotope probing (SIP) methods involving an effective labeling strategy using 13C-labeled dissolved inorganic carbon (DIC) as carbon source along with methanol as dissimilatory substrate.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbon Isotopes
/
Archaea
/
DNA, Archaeal
/
RNA, Archaeal
/
Isotope Labeling
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
Germany