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DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea.
Yin, Xiuran; Kulkarni, Ajinkya C; Friedrich, Michael W.
Affiliation
  • Yin X; Microbial Ecophysiology Group, Faculty of Biology/Chemistry, University of Bremen, Bremen, Germany.
  • Kulkarni AC; MARUM-Center for Marine Environmental Sciences, University of Bremen, Bremen, Germany.
  • Friedrich MW; International Max Planck Research School for Marine Microbiology, Max Planck Institute for Marine Microbiology, Bremen, Germany.
Methods Mol Biol ; 2046: 189-206, 2019.
Article in En | MEDLINE | ID: mdl-31407306
ABSTRACT
Methylotrophic methanogenic archaea are an integral part of the carbon cycle in various anaerobic environments. Different from methylotrophic bacteria, methylotrophic methanogens assimilate both, the methyl compound and dissolved inorganic carbon. Here, we present DNA- and RNA-stable isotope probing (SIP) methods involving an effective labeling strategy using 13C-labeled dissolved inorganic carbon (DIC) as carbon source along with methanol as dissimilatory substrate.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carbon Isotopes / Archaea / DNA, Archaeal / RNA, Archaeal / Isotope Labeling Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: Germany
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carbon Isotopes / Archaea / DNA, Archaeal / RNA, Archaeal / Isotope Labeling Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: Germany