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Generation and characterization of a human induced pluripotent stem cell line UOWi005-A from dermal fibroblasts derived from a CCNFS621G familial amyotrophic lateral sclerosis patient using mRNA reprogramming.
Bax, Monique; Balez, Rachelle; Muñoz, Sonia Sanz; Do-Ha, Dzung; Stevens, Claire H; Berg, Tracey; Cabral-da-Silva, Mauricio C; Engel, Martin; Nicholson, Garth; Yang, Shu; Blair, Ian P; Ooi, Lezanne.
Affiliation
  • Bax M; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Balez R; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Muñoz SS; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Do-Ha D; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Stevens CH; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Berg T; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Cabral-da-Silva MC; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Engel M; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia.
  • Nicholson G; University of Sydney ANZAC Research Institute, Concord Repatriation General Hospital, Sydney, Australia.
  • Yang S; Macquarie University Centre for Motor Neuron Disease Research, Macquarie University Faculty of Medicine and Health Sciences, NSW 2109, Australia.
  • Blair IP; Macquarie University Centre for Motor Neuron Disease Research, Macquarie University Faculty of Medicine and Health Sciences, NSW 2109, Australia.
  • Ooi L; Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia; School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, NSW 2522, Australia. Electronic address: lezanne@uow.edu.au.
Stem Cell Res ; 40: 101530, 2019 10.
Article in En | MEDLINE | ID: mdl-31445393
Dermal fibroblasts from a 59 year old male patient with amyotrophic lateral sclerosis (symptomatic at the time of collection), attributed to a mutation in the cyclin F gene (CCNFS621G), were reprogrammed using mRNA and microRNA-delivered OSKM factors to induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed pluripotent, expressing typical pluripotency markers and were capable of three germ layer differentiation. This is the first reported reprogramming of cells with a mutation in the cyclin F gene, and represents a novel resource for the study of amyotrophic lateral sclerosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cyclins / Dermis / Induced Pluripotent Stem Cells / Amyotrophic Lateral Sclerosis Limits: Humans / Male / Middle aged Language: En Journal: Stem Cell Res Year: 2019 Document type: Article Affiliation country: Australia Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cyclins / Dermis / Induced Pluripotent Stem Cells / Amyotrophic Lateral Sclerosis Limits: Humans / Male / Middle aged Language: En Journal: Stem Cell Res Year: 2019 Document type: Article Affiliation country: Australia Country of publication: United kingdom