Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae.
J Vet Med Sci
; 81(10): 1400-1408, 2019 Oct 18.
Article
in En
| MEDLINE
| ID: mdl-31462609
ABSTRACT
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacterial Proteins
/
Tuberculosis, Bovine
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Mycobacterium bovis
Type of study:
Diagnostic_studies
/
Guideline
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Vet Med Sci
Journal subject:
MEDICINA VETERINARIA
Year:
2019
Document type:
Article
Affiliation country:
Brazil