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Cold Atmospheric Pressure Plasma Jet Reduces Trichophyton rubrum Adherence and Infection Capacity.
Borges, Aline Chiodi; Nishime, Thalita Mayumi Castaldelli; de Moura Rovetta, Sabrina; Lima, Gabriela de Morais Gouvêa; Kostov, Konstantin Georgiev; Thim, Gilmar Patrocínio; de Menezes, Beatriz Rossi Canuto; Machado, João Paulo Barros; Koga-Ito, Cristiane Yumi.
Affiliation
  • Borges AC; Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University (UNESP), Avenida Engenheiro Francisco José Longo, 777, São José dos Campos, SP, 12245-000, Brazil.
  • Nishime TMC; Departament of Pharmacy, Taubaté Institute of Higher Education (ITES), Avenida Dom Pedro I, 3575, Taubaté, SP, 12090-000, Brazil.
  • de Moura Rovetta S; Department of Chemistry and Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (UNESP), Av. Dr. Ariberto Pereira da Cunha, 333, Guaratinguetá, SP, 12516-410, Brazil.
  • Lima GMG; Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Street 2, 17489, Greifswald, Germany.
  • Kostov KG; Departament of Pharmacy, Taubaté Institute of Higher Education (ITES), Avenida Dom Pedro I, 3575, Taubaté, SP, 12090-000, Brazil.
  • Thim GP; Departament of Pharmacy, Taubaté Institute of Higher Education (ITES), Avenida Dom Pedro I, 3575, Taubaté, SP, 12090-000, Brazil.
  • de Menezes BRC; Department of Chemistry and Physics, Guaratinguetá Faculty of Engineering, São Paulo State University (UNESP), Av. Dr. Ariberto Pereira da Cunha, 333, Guaratinguetá, SP, 12516-410, Brazil.
  • Machado JPB; Division of Fundamental Sciences, Instituto Tecnológico de Aeronáutica (ITA), Praça Marechal Eduardo Gomes, 50, São José dos Campos, SP, 12228-970, Brazil.
  • Koga-Ito CY; Division of Fundamental Sciences, Instituto Tecnológico de Aeronáutica (ITA), Praça Marechal Eduardo Gomes, 50, São José dos Campos, SP, 12228-970, Brazil.
Mycopathologia ; 184(5): 585-595, 2019 Oct.
Article in En | MEDLINE | ID: mdl-31471824
This study aimed to evaluate the effects of cold atmospheric pressure plasma (CAPP) jet on Trichophyton rubrum growth, germination and adherence to nail. The effects of plasma jet on T. rubrum conidia germination and on mycelial growth were evaluated by in vitro assays. An ex vivo nail infection model was used to evaluate the effects on conidia adherence and infection. Biochemical analyses of nail fragments exposed or not to CAPP were performed by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Plasma jet exposure for 10 and 15 min completely inhibited mycelial growth after only one exposure. Fifteen minutes of exposure could reduce conidia germination in suspension. Fungal suspensions exposed to plasma jet for 10 and 15 min were not able to infect nail specimens. These results were corroborated by ATR-FTIR analyses of nail fragments. In conclusion, single exposure to CAPP for 15 min was able to inhibit fungal growth, adherence and infection capacity. The results suggest that cold atmospheric plasma jet can be a promising alternative for the treatment of onychomycoses caused by T. rubrum.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Atmospheric Pressure / Tinea / Trichophyton / Cell Adhesion / Plasma Gases Limits: Humans Language: En Journal: Mycopathologia Year: 2019 Document type: Article Affiliation country: Brazil Country of publication: Netherlands
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Atmospheric Pressure / Tinea / Trichophyton / Cell Adhesion / Plasma Gases Limits: Humans Language: En Journal: Mycopathologia Year: 2019 Document type: Article Affiliation country: Brazil Country of publication: Netherlands