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Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye.
Perlikowski, Dawid; Wisniewska, Halina; Góral, Tomasz; Ochodzki, Piotr; Majka, Maciej; Pawlowicz, Izabela; Belter, Jolanta; Kosmala, Arkadiusz.
Affiliation
  • Perlikowski D; Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland.
  • Wisniewska H; Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland.
  • Góral T; Plant Breeding and Acclimatization Institute - National Research Institute, 05-870 Blonie, Poland.
  • Ochodzki P; Plant Breeding and Acclimatization Institute - National Research Institute, 05-870 Blonie, Poland.
  • Majka M; Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland.
  • Pawlowicz I; Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland.
  • Belter J; Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland.
  • Kosmala A; Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland.
Plant Pathol J ; 35(4): 313-320, 2019 Aug.
Article in En | MEDLINE | ID: mdl-31481854
ABSTRACT
Rye was used here to dissect molecular mechanisms of resistance to Fusarium head blight (FHB) and to go deeper with our understanding of that process in cereals. F. culmorum-damaged kernels of two lines different in their potential of resistance to FHB were analyzed using two-dimensional gel electrophoresis and mass spectrometry to identify resistance markers. The proteome profiling was accompanied by measurements of α- and ß-amylase activities and mycotoxin content. The proteomic studies indicated a total of 18 spots with clear differences in protein abundance between the more resistant and more susceptible rye lines after infection. Eight proteins were involved in carbohydrate metabolism of which six proteins showed a significantly higher abundance in the resistant line. The other proteins recognized here were involved in stress response and redox homeostasis. Three remaining proteins were associated with protease inhibition/resistance and lignin biosynthesis, revealing higher accumulation levels in the susceptible rye line. After inoculation, the activities of α- and ß-amylases, higher in the susceptible line, were probably responsible for a higher level of starch decomposition after infection and a higher susceptibility to FHB. The presented results could be a good reference for further research to improve crop resistance to FHB.
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies / Risk_factors_studies Language: En Journal: Plant Pathol J Year: 2019 Document type: Article Affiliation country: Poland

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Diagnostic_studies / Risk_factors_studies Language: En Journal: Plant Pathol J Year: 2019 Document type: Article Affiliation country: Poland