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Human Intestinal Enteroids Model MHC-II in the Gut Epithelium.
Wosen, Jonathan E; Ilstad-Minnihan, Alexandra; Co, Julia Y; Jiang, Wei; Mukhopadhyay, Dhriti; Fernandez-Becker, Nielsen Q; Kuo, Calvin J; Amieva, Manuel R; Mellins, Elizabeth D.
Affiliation
  • Wosen JE; Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Ilstad-Minnihan A; Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Co JY; Division of Infectious Diseases, Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Jiang W; Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Mukhopadhyay D; Program in Immunology, Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Fernandez-Becker NQ; Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University, Stanford, CA, United States.
  • Kuo CJ; Division of Hematology, Department of Medicine, Stanford University, Stanford, CA, United States.
  • Amieva MR; Division of Infectious Diseases, Department of Pediatrics, Stanford University, Stanford, CA, United States.
  • Mellins ED; Department of Microbiology and Immunology, Stanford University, Stanford, CA, United States.
Front Immunol ; 10: 1970, 2019.
Article in En | MEDLINE | ID: mdl-31481960
ABSTRACT
The role of intestinal epithelial cells (IECs) in mucosal tolerance and immunity remains poorly understood. We present a method for inducing MHC class II (MHC-II) in human enteroids, "mini-guts" derived from small intestinal crypt stem cells, and show that the intracellular MHC-II peptide-pathway is intact and functional in IECs. Our approach enables human enteroids to be used for novel in vitro studies into IEC MHC-II regulation and function during health and disease.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoids / Histocompatibility Antigens Class II / Intestinal Mucosa Limits: Humans Language: En Journal: Front Immunol Year: 2019 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organoids / Histocompatibility Antigens Class II / Intestinal Mucosa Limits: Humans Language: En Journal: Front Immunol Year: 2019 Document type: Article Affiliation country: United States