miR5191 functions as a tumor suppressor by targeting RPS6KB1 in colorectal cancer.
Int J Oncol
; 2019 Aug 30.
Article
in En
| MEDLINE
| ID: mdl-31485593
ABSTRACT
MicroRNAs (miRNAs/miRs) are a class of small noncoding RNAs that play pivotal roles in cancer physiology as important epigenetic regulators of gene expression. Several miRNAs have been previously discovered that regulate the proliferation of the colorectal cancer (CRC) cell line HCT116. In the present study, one of these miRNAs, miR5191, was characterized as a tumor suppressor in CRC cells. Transfection with miR5191 led to a significant decrease in cell proliferation, invasiveness, tumor sphereforming ability and tumor organoid growth, as determined via trypan blue, Transwell, sphere culture and organoid culture assays, respectively. Flow cytometric analyses revealed that miR5191 induced the cell cycle arrest and apoptosis of CRC cells. Additionally, the expression of miR5191 was downregulated in CRC tumor tissues compared with in normal tissues, as measured by reverse transcriptionquantitative PCR analysis. Ribosomal protein S6 kinase ß1 (RPS6KB1) was identified as a direct target of miR5191. Ectopic expression of RPS6KB1 suppressed the function of miR5191. Intratumoral injection of miR5191 mimic suppressed tumor growth in HCT116 xenografts. These findings suggested a novel tumorsuppressive function for miR5191 in CRC, and its potential applicability for the development of anticancer miRNA therapeutics.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Prognostic_studies
Language:
En
Journal:
Int J Oncol
Journal subject:
NEOPLASIAS
Year:
2019
Document type:
Article