Development and validation of a pen side test for Rift Valley fever.
PLoS Negl Trop Dis
; 13(9): e0007700, 2019 09.
Article
in En
| MEDLINE
| ID: mdl-31509527
ABSTRACT
BACKGROUND:
Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures. METHODOLOGY/PRINCIPALFINDINGS:
A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera. CONCLUSION/SIGNIFICANCE:
The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Rift Valley Fever
/
Rift Valley fever virus
/
Immunoassay
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
PLoS Negl Trop Dis
Journal subject:
MEDICINA TROPICAL
Year:
2019
Document type:
Article
Affiliation country:
France