TcTASV Antigens of Trypanosoma cruzi: Utility for Diagnosis and High Accuracy as Biomarkers of Treatment Efficacy in Pediatric Patients.
Am J Trop Med Hyg
; 101(5): 1135-1138, 2019 11.
Article
in En
| MEDLINE
| ID: mdl-31516110
ABSTRACT
The discovery and characterization of novel parasite antigens to improve the diagnosis of Trypanosoma cruzi by serological methods and for accurate and rapid follow-up of treatment efficiency are still needed. TcTASV is a T. cruzi-specific multigene family, whose products are expressed on the parasite stages present in the vertebrate host. In a previous work, a mix of antigens from subfamilies TcTASV-A and TcTASV-C (Mix A + C) was sensitive and specific to identify dogs with active infection of high epidemiological relevance. Here, TcTASV-A and TcTASV-C were assayed separately as well as together (Mix A + C) in an ELISA format on human samples. The Mix A + C presented moderate sensitivity (78%) but high diagnostic accuracy with a 100% of specificity, evaluated on healthy, leishmaniasic, and Strongyloides stercoralis infected patients. Moreover, antibody levels of pediatric patients showed-2 years posttreatment-diminished reactivity against the Mix A + C (P < 0.0001), pointing TcTASV antigens as promising tools for treatment follow-up.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trypanosoma cruzi
/
Chagas Disease
/
Antigens, Protozoan
/
Antiprotozoal Agents
Type of study:
Diagnostic_studies
Limits:
Child
/
Humans
Language:
En
Journal:
Am J Trop Med Hyg
Year:
2019
Document type:
Article
Affiliation country:
Argentina