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IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis using Taenia crassiceps hydrophobic antigens.
Carrara, Guilherme M P; Silva, Gabriela B; Faria, Lucas S; Nunes, Daniela S; Ribeiro, Vanessa S; Lopes, Camila A; Gonçalves-Pires, Maria do Rosário F; Santos, Malu M; Borges, Isabela P; Ferreira-Junior, Álvaro; Ávila, Veridiana M R; Costa-Cruz, Julia M.
Affiliation
  • Carrara GMP; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Silva GB; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Faria LS; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Nunes DS; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Ribeiro VS; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Lopes CA; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Gonçalves-Pires MDRF; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
  • Santos MM; Programa de Pós-Graduação em Sanidade e Produção Animal nos Trópicos, Universidade de Uberaba, Campus Aeroporto. Av. Nenê Sabino, sala 2D05, Uberaba, Minas Gerais38055-500, Brasil.
  • Borges IP; Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, Minas Gerais38400-902, Brasil.
  • Ferreira-Junior Á; Programa de Pós-Graduação em Sanidade e Produção Animal nos Trópicos, Universidade de Uberaba, Campus Aeroporto. Av. Nenê Sabino, sala 2D05, Uberaba, Minas Gerais38055-500, Brasil.
  • Ávila VMR; Laboratório de Bioquímica e Toxinas Animais, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Av. Pará 1720, Uberlândia, Minas Gerais38400-902, Brasil.
  • Costa-Cruz JM; Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Av. Amazonas s/n, bloco 4C, Uberlândia, MinasGerais38400-902, Brasil.
Parasitology ; 147(2): 240-247, 2020 02.
Article in En | MEDLINE | ID: mdl-31603062
ABSTRACT
Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Taenia / Immunologic Tests / Immunoglobulins / Neurocysticercosis / Antigens, Helminth Type of study: Diagnostic_studies Limits: Animals / Female / Humans Language: En Journal: Parasitology Year: 2020 Document type: Article Affiliation country: Brazil Publication country: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM
Fulltext
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Print
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Taenia / Immunologic Tests / Immunoglobulins / Neurocysticercosis / Antigens, Helminth Type of study: Diagnostic_studies Limits: Animals / Female / Humans Language: En Journal: Parasitology Year: 2020 Document type: Article Affiliation country: Brazil Publication country: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM