Improved Nanoflow RPLC-CZE-MS/MS System with High Peak Capacity and Sensitivity for Nanogram Bottom-up Proteomics.

Yang, Zhichang; Shen, Xiaojing; Chen, Daoyang; Sun, Liangliang

Yang, Zhichang; Shen, Xiaojing; Chen, Daoyang; Sun, Liangliang.

Affiliation

Yang Z; Department of Chemistry , Michigan State University , 578 S Shaw Lane , East Lansing , Michigan 48824 , United States.
Shen X; Department of Chemistry , Michigan State University , 578 S Shaw Lane , East Lansing , Michigan 48824 , United States.
Chen D; Department of Chemistry , Michigan State University , 578 S Shaw Lane , East Lansing , Michigan 48824 , United States.
Sun L; Department of Chemistry , Michigan State University , 578 S Shaw Lane , East Lansing , Michigan 48824 , United States.

J Proteome Res ; 18(11): 4046-4054, 2019 11 01.

Article in En | MEDLINE | ID: mdl-31610113

Subject(s)

Chromatography, Reverse-Phase/methods; Electrophoresis, Capillary/methods; Nanotechnology/methods; Proteomics/methods; Tandem Mass Spectrometry/methods; Animals; Cattle; HEK293 Cells; Humans; MCF-7 Cells; Peptides/chemistry; Peptides/metabolism; Proteome/chemistry; Proteome/metabolism; Reproducibility of Results; Serum Albumin, Bovine/chemistry

Key words

CZE-MS; LC-CZE; bottom-up proteomics; high peak capacity; high sensitivity; mass-limited sample; single-spot solid-phase sample preparation (SP3)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Electrophoresis, Capillary / Nanotechnology / Proteomics / Tandem Mass Spectrometry / Chromatography, Reverse-Phase Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Humans Language: En Journal: J Proteome Res Journal subject: BIOQUIMICA Year: 2019 Document type: Article Affiliation country: United States Country of publication: United States

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