Effects of anticoagulants on stable-isotope values (δ13 C and δ15 N) of shark blood components.
J Fish Biol
; 95(6): 1535-1539, 2019 Dec.
Article
in En
| MEDLINE
| ID: mdl-31621068
ABSTRACT
The effects of anticoagulant EDTA and sodium heparin (SH) on stable carbon δ13 C and nitrogen δ15 N isotopic values of red blood cells (RBC) and blood plasma in juvenile blacktip reef sharks Carcharhinus melanopterus were analysed. Plasma preserved with anticoagulants was not isotopically distinct from plasma stored in no-additive control tubes but RBC δ15 N values exhibited small enrichments when preserved with EDTA and SH. Results suggest EDTA and SH are viable anticoagulants for stable isotopic analyses of blood fractions but further studies are advised to validate results.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasma
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Sharks
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Carbon Isotopes
/
Erythrocytes
/
Anticoagulants
/
Nitrogen Isotopes
Limits:
Animals
Language:
En
Journal:
J Fish Biol
Year:
2019
Document type:
Article
Affiliation country:
France
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
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UK
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UNITED KINGDOM