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Stain-Free Resolution of Unmyelinated Axons in Transgenic Mice Using Fluorescence Microscopy.
Mohan, Suresh; Coto Hernández, Iván; Selig, Martin K; Shibata, Shinsuke; Jowett, Nate.
Affiliation
  • Mohan S; Surgical Photonics and Engineering Laboratory, Department of Otolaryngology - Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School.
  • Coto Hernández I; Diagnostic Electron Microscopy Unit, Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts.
  • Selig MK; Electron Microscope Laboratory, Keio University School of Medicine, Tokyo, Japan.
  • Shibata S; Surgical Photonics and Engineering Laboratory, Department of Otolaryngology - Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School.
  • Jowett N; Surgical Photonics and Engineering Laboratory, Department of Otolaryngology - Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School.
J Neuropathol Exp Neurol ; 78(12): 1178-1180, 2019 12 01.
Article in En | MEDLINE | ID: mdl-31642916
ABSTRACT
Though unmyelinated fibers predominate axon counts within peripheral nerves, they are frequently excluded in histomorphometric assessment as they cannot be readily resolved by light microscopy. Herein, we demonstrate stain-free resolution of unmyelinated axons in Sox10-Venus mice by widefield fluorescence imaging of sciatic nerve cryosections. Optional staining of cryosections using a rapid and nontoxic myelin-specific dye (FluoroMyelin Red) enables robust synchronous resolution of myelinated and unmyelinated fibers, comprising a high-throughput platform for neural histomorphometry.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sciatic Nerve / Axons / Nerve Fibers, Unmyelinated / Microscopy, Fluorescence Limits: Animals Language: En Journal: J Neuropathol Exp Neurol Year: 2019 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sciatic Nerve / Axons / Nerve Fibers, Unmyelinated / Microscopy, Fluorescence Limits: Animals Language: En Journal: J Neuropathol Exp Neurol Year: 2019 Document type: Article