Ribosomal protein uL3 targets E2F1 and Cyclin D1 in cancer cell response to nucleolar stress.
Sci Rep
; 9(1): 15431, 2019 10 28.
Article
in En
| MEDLINE
| ID: mdl-31659203
ABSTRACT
Several experimental strategies in the treatment of cancer include drug alteration of cell cycle regulatory pathways as a useful strategy. Extra-ribosomal functions of human ribosomal protein L3 (uL3) may affect DNA repair, cell cycle arrest and apoptosis. In the present study, we demonstrated that uL3 is required for the activation of G1/S transition genes. Luciferase assays established that uL3 negatively regulates the activity of E2F1 promoter. Induced ribosome-free uL3 reduces Cyclin D1 mRNA and protein levels. Using protein/protein immunoprecipitation methods, we demonstrated that uL3 physically interacts with PARP-1 affecting E2F1 transcriptional activity. Our findings led to the identification of a new pathway mediated by uL3 involving E2F1 and Cyclin D1 in the regulation of cell cycle progression.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribosomal Proteins
/
Stress, Physiological
/
Cell Nucleolus
/
Cyclin D1
/
E2F1 Transcription Factor
/
Neoplasms
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Sci Rep
Year:
2019
Document type:
Article
Affiliation country:
Italy