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CReasPy-Cloning: A Method for Simultaneous Cloning and Engineering of Megabase-Sized Genomes in Yeast Using the CRISPR-Cas9 System.
Ruiz, Estelle; Talenton, Vincent; Dubrana, Marie-Pierre; Guesdon, Gabrielle; Lluch-Senar, Maria; Salin, Franck; Sirand-Pugnet, Pascal; Arfi, Yonathan; Lartigue, Carole.
Affiliation
  • Ruiz E; INRA , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Talenton V; Univ. Bordeaux , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Dubrana MP; INRA , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Guesdon G; Univ. Bordeaux , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Lluch-Senar M; INRA , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Salin F; Univ. Bordeaux , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Sirand-Pugnet P; INRA , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Arfi Y; Univ. Bordeaux , UMR 1332 de Biologie du Fruit et Pathologie , F-33140 Villenave d'Ornon , France.
  • Lartigue C; EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG) , The Barcelona Institute of Science and Technology , Dr Aiguader 88 , Barcelona 08003 , Spain.
ACS Synth Biol ; 8(11): 2547-2557, 2019 11 15.
Article in En | MEDLINE | ID: mdl-31663334
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Genetic Engineering / Cloning, Molecular / CRISPR-Cas Systems / Gene Editing Language: En Journal: ACS Synth Biol Year: 2019 Document type: Article Affiliation country: France Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Saccharomyces cerevisiae / Genetic Engineering / Cloning, Molecular / CRISPR-Cas Systems / Gene Editing Language: En Journal: ACS Synth Biol Year: 2019 Document type: Article Affiliation country: France Country of publication: United States