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Abnormal retinal pigment epithelium melanogenesis as a major determinant for radiation-induced congenital eye defects.
Craenen, Kai; Verslegers, Mieke; Craeghs, Livine; Quintens, Roel; Janssen, Ann; Coolkens, Amelie; Baatout, Sarah; Moons, Lieve; Benotmane, Mohammed Abderrafi.
Affiliation
  • Craenen K; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium; Laboratory of Neural Circuit Development and Regeneration, Animal Physiology and Neurobiology section, Department of Biology, Fac
  • Verslegers M; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium.
  • Craeghs L; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium; Laboratory of Neural Circuit Development and Regeneration, Animal Physiology and Neurobiology section, Department of Biology, Fac
  • Quintens R; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium.
  • Janssen A; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium.
  • Coolkens A; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium.
  • Baatout S; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium.
  • Moons L; Laboratory of Neural Circuit Development and Regeneration, Animal Physiology and Neurobiology section, Department of Biology, Faculty of Science, KU Leuven, Naamsestraat 61 bus 2464, Leuven 3000, Belgium.
  • Benotmane MA; Radiobiology Unit, Interdisciplinary Biosciences, Institute for Environment, Health and Safety, Belgian Nuclear Research Centre SCK-CEN, Boeretang 200, Mol 2400, Belgium. Electronic address: rafi.benotmane@SCKCEN.be.
Reprod Toxicol ; 91: 59-73, 2020 01.
Article in En | MEDLINE | ID: mdl-31705956
Recent studies highlighted a link between ionizing radiation exposure during neurulation and birth defects such as microphthalmos and anophthalmos. Because the mechanisms underlying these defects remain largely unexplored, we irradiated pregnant C57BL/6J mice (1.0 Gy, X-rays) at embryonic day (E)7.5, followed by histological and gene/protein expression analyses at defined days. Irradiation impaired embryonic development at E9 and we observed a delayed pigmentation of the retinal pigment epithelium (RPE) at E11. In addition, a reduced RNA expression and protein abundance of critical eye-development genes (e.g. Pax6 and Lhx2) was observed. Furthermore, a decreased expression of Mitf, Tyr and Tyrp1 supported the radiation-induced perturbation in RPE pigmentation. Finally, via immunostainings for proliferation (Ki67) and mitosis (phosphorylated histone 3), a decreased mitotic index was observed in the E18 retina after exposure at E7.5. Overall, we propose a plausible etiological model for radiation-induced eye-size defects, with RPE melanogenesis as a major determining factor.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Radiation Injuries, Experimental / X-Rays / Retinal Pigment Epithelium / Melanins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Reprod Toxicol Journal subject: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Year: 2020 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Radiation Injuries, Experimental / X-Rays / Retinal Pigment Epithelium / Melanins Type of study: Prognostic_studies Limits: Animals Language: En Journal: Reprod Toxicol Journal subject: EMBRIOLOGIA / MEDICINA REPRODUTIVA / TOXICOLOGIA Year: 2020 Document type: Article Country of publication: United States