A High-Throughput Strategy for Recombinant Protein Expression and Solubility Screen in Escherichia coli : A Case of Sensor Histidine Kinase.

Szmitkowska, Agnieszka; Pekárová, Blanka; Hejátko, Jan

Szmitkowska, Agnieszka; Pekárová, Blanka; Hejátko, Jan.

Affiliation

Szmitkowska A; Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic.
Pekárová B; Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic.
Hejátko J; Central European Institute of Technology and National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic. hejatko@sci.muni.cz.

Methods Mol Biol ; 2077: 19-36, 2020.

Article in En | MEDLINE | ID: mdl-31707649

Subject(s)

Escherichia coli/genetics; Gene Expression; High-Throughput Screening Assays; Histidine Kinase/genetics; Recombinant Proteins/genetics; Escherichia coli/metabolism; Histidine Kinase/chemistry; Histidine Kinase/isolation & purification; Histidine Kinase/metabolism; Phosphorylation; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Solubility

Key words

Expression screen; Growth conditions; Histidine kinase; Protein; Sample preparation; Solubility screen

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Gene Expression / Escherichia coli / High-Throughput Screening Assays / Histidine Kinase Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2020 Document type: Article Affiliation country: Czech Republic Country of publication: United States

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