X-irradiation of developing hippocampal neurons causes changes in neuron population phenotypes, dendritic morphology and synaptic protein expression in surviving neurons at maturity.
Neurosci Res
; 160: 11-24, 2020 Nov.
Article
in En
| MEDLINE
| ID: mdl-31711782
ABSTRACT
The effects of X-irradiation on developing neurons and their functions are unclear. We used primary cultures of mouse hippocampal neurons to investigate the effects of X-irradiation on cell death in developing neurons by analyzing caspase-3, MAP2 and DAPI-labeled cells, and the phenotypes and function of surviving neurons, by examining GAD67-positive cells as a GABAergic marker, and the synaptic markers synapsin 1, drebrin and PSD-95 through its maturation. One-day in vitro (DIV 1) cells were exposed to 0.5â¯Gy or 1â¯Gy of X-rays. A significant increase in the percentage of activated caspase-3, a decrease in the number of MAP2/DAPI-positive cells and change in the percentage of GAD67 positive neurons, compared with sham controls, were found 6 days after 1â¯Gy and 13 days after 0.5â¯Gy of X-rays. The expression of PSD-95 and drebrin, as well as drebrin clusters, in the remaining neurons was decreased at DIV 21, in both 0.5â¯Gy and on 1â¯Gy-irradiation there was a reduced number of dendritic intersection as well. Together, our findings show that 0.5â¯Gy and 1â¯Gy of X-irradiation at DIV 1 not only causes neuronal cell death but elicits an increase in the percentage of inhibitory neurons, changes in the dendrites and decrease in expression of important synaptic proteins in the surviving neurons at maturity 3 weeks after exposure.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hippocampus
/
Neurons
Type of study:
Etiology_studies
Limits:
Animals
Language:
En
Journal:
Neurosci Res
Journal subject:
NEUROLOGIA
Year:
2020
Document type:
Article