Protective effect of seleno-amino-oligosaccharide on oxidative damage of IPEC-1 cells by activating Keap1/Nrf2 signaling pathway.
Int J Biol Macromol
; 155: 972-978, 2020 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-31712138
ABSTRACT
The aim of the study was to investigate the antioxidant effect of seleno-amino-oligosaccharide (Se-AOS) on intestinal porcine epithelial cells (IPEC-1). MTT assay showed that Se-AOS had no effect on the viability of IPEC-1 cells up to a concentration of 9200⯵g/L and Se-AOS significantly increased the viability of IPEC-1 cells compared to cells exposed to H2O2 alone. Se-AOS significantly increased the level of superoxide Dismutase (SOD) and decreased the levels of malonic dialdehyde (MDA) and lactate dehydrogenase (LDH) in IPEC-1 cells. The gene expression levels of different antioxidant enzymes dramatically increased by the pretreatment of Se-AOS compared to H2O2 treatment. In addition, the results indicated that Se-AOS up-regulated the intracellular Nrf2 and down-regulated the level of Keap1 by western blot. Taken together, these findings suggested that Se-AOS can protect IPEC-1 cells from oxidative damage through activating the Keap1/Nrf2 signaling pathway.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligosaccharides
/
Selenium
/
Signal Transduction
/
Cell Survival
/
Epithelial Cells
/
Antioxidants
Limits:
Animals
Language:
En
Journal:
Int J Biol Macromol
Year:
2020
Document type:
Article