Effect of blood contamination of cerebrospinal fluid on amino acids, biogenic amines, pterins and vitamins.
Fluids Barriers CNS
; 16(1): 34, 2019 Nov 14.
Article
in En
| MEDLINE
| ID: mdl-31727079
ABSTRACT
BACKGROUND:
Cerebrospinal fluid (CSF) metabolomic investigations are a powerful tool for studying neurometabolic diseases. We aimed to assess the effect of CSF contamination with blood on the concentrations of selected biomarkers.METHODS:
CSF samples were spiked in duplicate with increasing volumes of whole blood under two conditions (A) pooled CSF spiked with fresh whole blood and frozen to cause red blood cell (RBC) lysis; (B) pooled CSF spiked with fresh blood and centrifuged (the supernatant with no RBCs was frozen until the moment of analysis). CSF concentrations of amino acids, biogenic amines, pterins, and vitamins were analysed by HPLC coupled with tandem mass spectrometry, electrochemical and fluorescence detection.RESULTS:
Aspartate, glutamate, taurine, ornithine, glycine, citrulline, pyridoxal 5´-phosphate, 5-methyltetrahydrofolate, and thiamine showed higher values when RBCs were lysed when compared with those of CSF with no RBC, while arginine, 5-hydroxyindoleacetic and homovanillic acids showed lower values. When RBCs were removed from CSF, only some amino acids, thiamine and pyridoxal 5´-phosphate showed moderately higher values when compared with the non-spiked CSF sample.CONCLUSIONS:
CSF-targeted metabolomic analysis is feasible even when substantial RBC contamination of CSF has occurred since CSF centrifugation to remove RBC prior to freezing eliminated most of the interferences observed.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pterins
/
Vitamins
/
Biogenic Amines
/
Blood Chemical Analysis
/
Cerebrospinal Fluid
Limits:
Humans
Language:
En
Journal:
Fluids Barriers CNS
Year:
2019
Document type:
Article
Affiliation country:
Spain