Novel Convenient Approach to the Solid-Phase Synthesis of Oligonucleotide Conjugates.
Molecules
; 24(23)2019 Nov 22.
Article
in En
| MEDLINE
| ID: mdl-31771111
ABSTRACT
A novel and convenient approach for the solid-phase 5'-functionalization of oligonucleotides is proposed in this article. The approach is based on the activation of free 5'-hydroxyl of polymer support-bound protected oligonucleotides by N,N'-disuccinimidyl carbonate followed by interaction with amino-containing ligands. Novel amino-containing derivatives of closo-dodecaborate, estrone, cholesterol, and α-tocopherol were specially prepared. A wide range of oligonucleotide conjugates bearing closo-dodecaborate, short peptide, pyrene, lipophilic residues (cholesterol, α-tocopherol, folate, estrone), aliphatic diamines, and propargylamine were synthesized and characterized to demonstrate the versatility of the approach. The developed method is suitable for the conjugate synthesis of oligonucleotides of different types (ribo-, deoxyribo-, 2'-O-methylribo-, and others).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligonucleotides
/
Solid-Phase Synthesis Techniques
Language:
En
Journal:
Molecules
Journal subject:
BIOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
RUSSIA