Recurrent laryngeal nerve regeneration using a self-assembling peptide hydrogel.
Laryngoscope
; 130(10): 2420-2427, 2020 10.
Article
in En
| MEDLINE
| ID: mdl-31804718
ABSTRACT
OBJECTIVES/HYPOTHESIS:
To regenerate defected recurrent laryngeal nerves (RLNs), various methods have been developed. However, no consistently effective treatments are currently available because of their insufficient functional recovery. RADA16-I, a self-assembling peptide used clinically as a hemostat, reportedly supports neurite outgrowth and functional synapse formation in vitro. The purpose of this study was to investigate the effect of RADA16-I hydrogels on transected RLNs in rats. STUDYDESIGN:
Animal experiments with controls.METHODS:
Fifteen adult rats were divided into the following three groups RADA16-I (+), RADA16-I (-), and neurectomy. A 6-mm gap of the left RLN was bridged using an 8-mm silicone tube in the RADA16-I (-) and RADA16-I (+) groups. Subsequently, RADA16-I hydrogel was injected into the tube in the RADA16-I (+) group. The surgical incisions were closed without any further treatment in the neurectomy group. After 8 weeks, laryngoscopy and electrophysiological and histological examinations were performed to evaluate the effect of RADA16-I on nerve regeneration and thyroarytenoid muscle atrophy.RESULTS:
Although most rats in the three groups exhibited no improvements of their vocal fold movement, partial recovery was observed in one rat in the RADA16-I (+) group. The neurofilament-positive areas and the number of myelinated nerves in the RADA16-I (+) group were significantly higher than in the RADA16-I (-) group. The area of the left thyroarytenoid muscle in the RADA16-I (+) group was significantly larger than that of the neurectomy group.CONCLUSIONS:
Our results suggested that RADA16-I hydrogel was effective for RLN regeneration. LEVEL OF EVIDENCE NA Laryngoscope, 1302420-2427, 2020.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Recurrent Laryngeal Nerve
/
Nerve Regeneration
Limits:
Animals
Language:
En
Journal:
Laryngoscope
Journal subject:
OTORRINOLARINGOLOGIA
Year:
2020
Document type:
Article
Affiliation country:
Japan