Adhesion G-protein-coupled receptor, GPR56, is required for Müllerian duct development in the chick.
J Endocrinol
; 244(2): 395-413, 2020 02.
Article
in En
| MEDLINE
| ID: mdl-31829965
ABSTRACT
The embryonic Müllerian ducts give rise to the female reproductive tract (fallopian tubes, uterus and upper vagina in humans, the oviducts in birds). Embryonic Müllerian ducts initially develop in both sexes, but later regress in males under the influence of anti-Müllerian hormone. While the molecular and endocrine control of duct regression in males have been well studied, early development of the ducts in both sexes is less well understood. Here, we describe a novel role for the adhesion G protein-coupled receptor, GPR56, in development of the Müllerian ducts in the chicken embryo. GPR56 is expressed in the ducts of both sexes from early stages. The mRNA is present during the elongation phase of duct formation, and it is restricted to the inner Müllerian duct epithelium. The putative ligand, Collagen III, is abundantly expressed in the Müllerian duct at the same developmental stages. Knockdown of GPR56 expression using in ovo electroporation results in variably truncated ducts, with a loss of expression of both epithelial and mesenchymal markers of duct development. Over-expression of GPR56 in vitro results in enhanced cell proliferation and cell migration. These results show that GPR56 plays an essential role in avian Müllerian duct development through the regulation of duct elongation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chickens
/
Avian Proteins
/
Receptors, G-Protein-Coupled
/
Mullerian Ducts
Limits:
Animals
Language:
En
Journal:
J Endocrinol
Year:
2020
Document type:
Article
Affiliation country:
Australia