Burn-related Collagen Conformational Changes in ex vivo Porcine Skin using Raman Spectroscopy.
Sci Rep
; 9(1): 19138, 2019 12 16.
Article
in En
| MEDLINE
| ID: mdl-31844072
ABSTRACT
This study utilizes Raman spectroscopy to analyze the burn-induced collagen conformational changes in ex vivo porcine skin tissue. Raman spectra of wavenumbers 500-2000 cm-1 were measured for unburnt skin as well as four different burn conditions (i) 200 °F for 10 s, (ii) 200 °F for the 30 s, (iii) 450 °F for 10 s and (iv) 450 °F for 30 s. The overall spectra reveal that protein and amino acids-related bands have manifested structural changes including the destruction of protein-related functional groups, and transformation from α-helical to disordered structures which are correlated with increasing burn severity. The deconvolution of the amide I region (1580-1720 cm-1) and the analysis of the sub-bands reveal a change of the secondary structure of the collagen from the α-like helix dominated to the ß-aggregate dominated one. Such conformational changes may explain the softening of mechanical response in burnt tissues reported in the literature.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Spectrum Analysis, Raman
/
Burns
/
Collagen
Limits:
Animals
Language:
En
Journal:
Sci Rep
Year:
2019
Document type:
Article
Affiliation country:
United States
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM