Metabolism of 17ß-estradiol by Novosphingobium sp. ES2-1 as probed via HRMS combined with 13C3-labeling.
J Hazard Mater
; 389: 121875, 2020 05 05.
Article
in En
| MEDLINE
| ID: mdl-31862352
ABSTRACT
This study investigated the biodegradation and metabolic mechanisms of 17ß-estradiol (E2) by Novosphingobium sp. ES2-1 isolated from the activated sludge in a domestic sewage treatment plant (STP). It could degrade 97.1% E2 (73.5 µmol/L) in 7 d with a biodegradation half-life of 1.29 d. E2 was initially converted to estrone (E1), then to 4-hydroxyestrone (4-OH-E1), before subsequent monooxygenation reactions cleaved 4-OH-E1 into a metabolite with long-chain ketones structure (metabolite P8). However, when 4-OH-E1 was cleaved through the 4,5-seco pathway, the resulting phenol ring cleavage product could randomly condense with NH3 to yield a pyridine derivative, accompanied by the uncertain loss of a carboxy group at C4 before the condensation. The derivative was further oxidized into the metabolites with both pyridine and long-chain ketones structure (metabolite N5) through a similar formation mechanism as for P8 performed. This research presents several novel metabolites and shows that E2 can be biodegraded into the metabolite with long-chain structure through three optional pathways, thereby reducing E2 contamination.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sphingomonadaceae
/
Estradiol
/
Estrogens
Language:
En
Journal:
J Hazard Mater
Journal subject:
SAUDE AMBIENTAL
Year:
2020
Document type:
Article
Affiliation country:
China