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Genetic variability of pig and human rotavirus group A isolates from Slovakia.
Salamunova, S; Jackova, A; Csank, T; Mandelik, R; Novotny, J; Beckova, Z; Helmova, L; Vilcek, S.
Affiliation
  • Salamunova S; Department of Epizootiology and Preventive Veterinary Medicine, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia.
  • Jackova A; Department of Epizootiology and Preventive Veterinary Medicine, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia.
  • Csank T; Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia.
  • Mandelik R; Department of Epizootiology and Preventive Veterinary Medicine, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia.
  • Novotny J; Clinic of Pigs, University of Veterinary Medicine and Pharmacy, Komenskeho 73, 041 81, Kosice, Slovakia.
  • Beckova Z; Department of Clinical Microbiology, F.D. Roosevelt University Hospital, Nám. L. Svobodu 1, 975 17, Banská Bystrica, Slovakia.
  • Helmova L; St. Elizabeth University of Health and Social sciences, Palackého 1, 810 00, Bratislava, Slovakia.
  • Vilcek S; Department of Clinical Microbiology, F.D. Roosevelt University Hospital, Nám. L. Svobodu 1, 975 17, Banská Bystrica, Slovakia.
Arch Virol ; 165(2): 463-470, 2020 Feb.
Article in En | MEDLINE | ID: mdl-31863266
ABSTRACT
The aim of this work was the genetic typing of RVA isolates originating from pigs and human patients in Slovakia. Seventy-eight rectal swabs from domestic pigs and 30 stool samples from humans were collected. The whole VP7 (G genotypes), VP6 (I genotypes) and partial VP4 (P genotypes) ORFs were amplified by RT-PCR. Genetic variability was higher amongst porcine sequences, where four G genotypes (G3, G4, G5, G11), two P genotypes (P[6], P[13]) and one I5 genotype were detected. Human RVA strains were represented by two G genotypes (G1, G3), two I genotypes (I1, I2), and one P genotype (P[8]). Genetic analysis did not show a relationship between Slovakian porcine and human RVA strains, but phylogenetic grouping of some Slovakian porcine sequences with Hungarian human sequences in both G and P genotypes was observed.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rotavirus Infections / Swine Diseases / Genetic Variation / Rotavirus Limits: Animals / Humans Country/Region as subject: Europa Language: En Journal: Arch Virol Year: 2020 Document type: Article Affiliation country: Slovakia
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rotavirus Infections / Swine Diseases / Genetic Variation / Rotavirus Limits: Animals / Humans Country/Region as subject: Europa Language: En Journal: Arch Virol Year: 2020 Document type: Article Affiliation country: Slovakia