A Renal-Clearable Macromolecular Reporter for Near-Infrared Fluorescence Imaging of Bladder Cancer.
Angew Chem Int Ed Engl
; 59(11): 4415-4420, 2020 03 09.
Article
in En
| MEDLINE
| ID: mdl-31876017
ABSTRACT
Bladder cancer (BC) is a prevalent disease with high morbidity and mortality; however, inâ
vivo optical imaging of BC remains challenging because of the lack of cancer-specific optical agents with high renal clearance. Herein, a macromolecular reporter (CyP1) was synthesized for real-time near-infrared fluorescence (NIRF) imaging and urinalysis of BC in living mice. Because of the high renal clearance (ca. 94 % of the injection dosage at 24â
h post-injection) and its cancer biomarker (APN=aminopeptidaseâ
N) specificity, CyP1 can be efficiently transported to the bladder and specially turn on its NIRF signal to report the detection of BC in living mice. Moreover, CyP1 can be used for optical urinalysis, permitting the exâ
vivo tracking of tumor progression for therapeutic evaluation and easy translation of CyP2 as an inâ
vitro diagnostic assay. This study not only provides new opportunities for non-invasive diagnosis of BC, but also reveals useful guidelines for the development of molecular reporters for the detection of bladder diseases.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Urinary Bladder Neoplasms
/
Biomarkers, Tumor
/
Macromolecular Substances
/
Fluorescent Dyes
/
Aminopeptidases
Type of study:
Guideline
Limits:
Animals
/
Humans
Language:
En
Journal:
Angew Chem Int Ed Engl
Year:
2020
Document type:
Article
Affiliation country:
Singapore