The progestin norethindrone alters growth, reproductive histology and gene expression in zebrafish (Danio rerio).
Chemosphere
; 242: 125285, 2020 Mar.
Article
in En
| MEDLINE
| ID: mdl-31896209
ABSTRACT
The aim of this study was to assess the long-term effects of synthetic progestin norethindrone (NET) on the growth, reproductive histology, and transcriptional expression profiles of genes associated with the hypothalamic-pituitary-gonadal (HPG) axis and germ cells in adult zebrafish. Adult zebrafish were exposed to 7, 84 and 810â¯ng/L NET for 90 days. The results showed that exposure to 810â¯ng/L NET caused a significant decrease in growth of females and males. The ovary weight and GSI was significantly reduced by NET at concentrations of 84 or 810â¯ng/L, which came along with the delay of ovary maturation in females. However, NET at all treatments resulted in acceleration of sperm maturation in males. In the ovaries of females, a strong inhibition of cyp19a1a gene was observed following exposure to NET at 810â¯ng/L. Similarly, NET at the highest treatment led to a significant down-regulation of cyp17, cyp19a1a, vasa, nanos1, dazl and dmc1 genes in the testes of males. Taken together, the overall results demonstrated that NET could impact growth and gonadal maturation, with significant alterations of transcriptional expression genes along HPG axis and germ cells.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Progestins
/
Water Pollutants, Chemical
/
Zebrafish
/
Gene Expression
/
Zebrafish Proteins
/
Norethindrone
Limits:
Animals
Language:
En
Journal:
Chemosphere
Year:
2020
Document type:
Article