Methods to edit T cells for cancer immunotherapy.
Methods Enzymol
; 631: 107-135, 2020.
Article
in En
| MEDLINE
| ID: mdl-31948544
ABSTRACT
This chapter summarizes the recent findings regarding the development of gene-editing technologies applied to adoptive T cell immunotherapy for cancer. The emerging tools and strategies described below enable the generation of a tailored system, establishing T cells as an advanced biomaterial with redirected specificities that are both programmable and inducible and establish long-term antitumor potential. These reviewed techniques and strategies are implemented with an optimized protocol for the generation of CAR-T cells using lentiviral gene transfer.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Genetic Therapy
/
Immunotherapy, Adoptive
/
Gene Editing
/
Neoplasms
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Enzymol
Year:
2020
Document type:
Article
Affiliation country:
France