Widespread activation of developmental gene expression characterized by PRC1-dependent chromatin looping.
Sci Adv
; 6(2): eaax4001, 2020 01.
Article
in En
| MEDLINE
| ID: mdl-31950077
ABSTRACT
Polycomb repressive complexes 1 and 2 have been historically described as transcriptional repressors, but recent reports suggest that PRC1 might also support activation, although the underlying mechanisms remain elusive. Here, we show that stage-specific PRC1 binding at a subset of active promoters and enhancers during Drosophila development coincides with the formation of three-dimensional (3D) loops, an increase in expression during development and repression in PRC1 mutants. Dissection of the dachshund locus indicates that PRC1-anchored loops are versatile architectural platforms that persist when surrounding genes are transcriptionally active and fine-tune their expression. The analysis of RING1B binding profiles and 3D contacts during neural differentiation in mice suggests that this role is conserved in mammals.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatin
/
Gene Expression Regulation, Developmental
/
Drosophila melanogaster
/
Genes, Developmental
/
Polycomb Repressive Complex 1
Limits:
Animals
Language:
En
Journal:
Sci Adv
Year:
2020
Document type:
Article
Affiliation country:
France