microRNA-137 downregulates MCL1 in ovarian cancer cells and mediates cisplatin-induced apoptosis.
Pharmacogenomics
; 21(3): 195-207, 2020 02.
Article
in En
| MEDLINE
| ID: mdl-31967512
ABSTRACT
Aim:
miR-137 is downregulated in various cancers; however, its function in ovarian cancer remains unclear.Methods:
The roles of miR-137 in apoptosis were accessed through IC50 values and DAPI assay. The regulation of MCL1 by miR-137 was investigated through luciferase reporter assay and immunoblot.Results:
miR-137 mimic could decrease the IC50 value of cisplatin and promote apoptosis in OVCAR3 ovarian cancer cells. Using luciferase assay, results on a panel of anti-apoptotic proteins, we identified MCL1 as a target for miR-137 and the results were confirmed using immunoblot. Finally, the underlying pathway in which miR-137 may be involved was investigated by transcriptome sequencing.Conclusion:
These results suggest that miR-137 downregulates MCL1 in ovarian cancer cells and mediates cisplatin-induced apoptosis.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ovarian Neoplasms
/
Gene Expression Regulation, Neoplastic
/
Apoptosis
/
MicroRNAs
/
Myeloid Cell Leukemia Sequence 1 Protein
/
Carcinoma, Ovarian Epithelial
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
Pharmacogenomics
Journal subject:
FARMACOLOGIA
/
GENETICA MEDICA
Year:
2020
Document type:
Article