Isolated Microspore Culture in Brassica napus.
Methods Mol Biol
; 2122: 269-282, 2020.
Article
in En
| MEDLINE
| ID: mdl-31975309
ABSTRACT
Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B. napus which includes the steps necessary to isolate and culture microspores, to induce microspore-derived embryos, to produce doubled haploid plants from them, as well as to check for the developmental stage of the microspores isolated, their viability, and the ploidy level of regenerated plantlets.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pollen
/
Brassica napus
/
Tissue Culture Techniques
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2020
Document type:
Article
Affiliation country:
Spain