A sensitive two-step stacking by coupling field-enhanced sample injection and micelle to cyclodextrin stacking for the determination of neutral analytes.
J Chromatogr A
; 1618: 460854, 2020 May 10.
Article
in En
| MEDLINE
| ID: mdl-31980258
ABSTRACT
To promote the detection sensitivity of neutral analytes, a sensitive two-step stacking strategy by coupling field-enhanced sample injection and micelle to cyclodextrin stacking in reverse migrating micelles MEKC has been established by selecting jujuboside A and B as models. The stacking mechanism, affecting factors as well as analytical performances of the proposed method were investigated. Compared with typical injection, the sensitivity enhancement factors of this method were up to 140- and 152-fold for jujuboside A and B, respectively. The enhancements were 8-10 times better than only single stacking strategy by MCDS. The LODs were 0.2-0.3 µg mL-1, interday and intraday%RSD were both lower than 4.3%, and a good linearity (r>0.999) was obtained. Feasibility in real samples analysis was evaluated by using Semen Ziziphi Spinosae dispensing granule and rat's urine. The results suggested that the strategy was simple, reliable, sensitive and promising for the analysis of neutral analytes in complex sample matrix.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, Micellar Electrokinetic Capillary
/
Cyclodextrins
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
J Chromatogr A
Year:
2020
Document type:
Article
Affiliation country:
China