Your browser doesn't support javascript.
loading
Robotic fluidic coupling and interrogation of multiple vascularized organ chips.
Novak, Richard; Ingram, Miles; Marquez, Susan; Das, Debarun; Delahanty, Aaron; Herland, Anna; Maoz, Ben M; Jeanty, Sauveur S F; Somayaji, Mahadevabharath R; Burt, Morgan; Calamari, Elizabeth; Chalkiadaki, Angeliki; Cho, Alexander; Choe, Youngjae; Chou, David Benson; Cronce, Michael; Dauth, Stephanie; Divic, Toni; Fernandez-Alcon, Jose; Ferrante, Thomas; Ferrier, John; FitzGerald, Edward A; Fleming, Rachel; Jalili-Firoozinezhad, Sasan; Grevesse, Thomas; Goss, Josue A; Hamkins-Indik, Tiama; Henry, Olivier; Hinojosa, Chris; Huffstater, Tessa; Jang, Kyung-Jin; Kujala, Ville; Leng, Lian; Mannix, Robert; Milton, Yuka; Nawroth, Janna; Nestor, Bret A; Ng, Carlos F; O'Connor, Blakely; Park, Tae-Eun; Sanchez, Henry; Sliz, Josiah; Sontheimer-Phelps, Alexandra; Swenor, Ben; Thompson, Guy; Touloumes, George J; Tranchemontagne, Zachary; Wen, Norman; Yadid, Moran; Bahinski, Anthony.
Affiliation
  • Novak R; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Ingram M; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Marquez S; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Das D; CFD Research Corporation, Huntsville, AL, USA.
  • Delahanty A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Herland A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Maoz BM; Division of Micro and Nanosystems, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Jeanty SSF; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Somayaji MR; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Burt M; Department of Biomedical Engineering and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel.
  • Calamari E; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Chalkiadaki A; Emulate, Inc., Boston, MA, USA.
  • Cho A; CFD Research Corporation, Huntsville, AL, USA.
  • Choe Y; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Chou DB; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Cronce M; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Dauth S; CFD Research Corporation, Huntsville, AL, USA.
  • Divic T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Fernandez-Alcon J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Ferrante T; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.
  • Ferrier J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • FitzGerald EA; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Fleming R; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Jalili-Firoozinezhad S; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Grevesse T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Goss JA; Emulate, Inc., Boston, MA, USA.
  • Hamkins-Indik T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Henry O; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Hinojosa C; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Huffstater T; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Jang KJ; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Kujala V; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Leng L; Department of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisboa, Portugal.
  • Mannix R; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Milton Y; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Nawroth J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Nestor BA; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Ng CF; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • O'Connor B; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Park TE; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Sanchez H; Emulate, Inc., Boston, MA, USA.
  • Sliz J; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Sontheimer-Phelps A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Swenor B; Emulate, Inc., Boston, MA, USA.
  • Thompson G; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Touloumes GJ; Disease Biophysics Group, Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA.
  • Tranchemontagne Z; Emulate, Inc., Boston, MA, USA.
  • Wen N; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
  • Yadid M; Emulate, Inc., Boston, MA, USA.
  • Bahinski A; Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
Nat Biomed Eng ; 4(4): 407-420, 2020 04.
Article in En | MEDLINE | ID: mdl-31988458
Organ chips can recapitulate organ-level (patho)physiology, yet pharmacokinetic and pharmacodynamic analyses require multi-organ systems linked by vascular perfusion. Here, we describe an 'interrogator' that employs liquid-handling robotics, custom software and an integrated mobile microscope for the automated culture, perfusion, medium addition, fluidic linking, sample collection and in situ microscopy imaging of up to ten organ chips inside a standard tissue-culture incubator. The robotic interrogator maintained the viability and organ-specific functions of eight vascularized, two-channel organ chips (intestine, liver, kidney, heart, lung, skin, blood-brain barrier and brain) for 3 weeks in culture when intermittently fluidically coupled via a common blood substitute through their reservoirs of medium and endothelium-lined vascular channels. We used the robotic interrogator and a physiological multicompartmental reduced-order model of the experimental system to quantitatively predict the distribution of an inulin tracer perfused through the multi-organ human-body-on-chips. The automated culture system enables the imaging of cells in the organ chips and the repeated sampling of both the vascular and interstitial compartments without compromising fluidic coupling.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Robotics / Cell Culture Techniques / Microfluidics / Lab-On-A-Chip Devices Type of study: Prognostic_studies Limits: Humans Language: En Journal: Nat Biomed Eng Year: 2020 Document type: Article Affiliation country: United States Country of publication: United kingdom
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Robotics / Cell Culture Techniques / Microfluidics / Lab-On-A-Chip Devices Type of study: Prognostic_studies Limits: Humans Language: En Journal: Nat Biomed Eng Year: 2020 Document type: Article Affiliation country: United States Country of publication: United kingdom