Development of a new chromogenic method for putrescine quantification using coupling reactions involving putrescine oxidase.
Anal Biochem
; 593: 113607, 2020 03 15.
Article
in En
| MEDLINE
| ID: mdl-32006471
ABSTRACT
Quantification of polyamines, including putrescine, is generally performed using high-performance liquid chromatography (HPLC) or gas chromatography. However, these methods are time-consuming because of sample derivatization and analytical reagent preparation. In this study, we developed a simple and high-throughput putrescine quantification method on a 96-well microtiter plate using putrescine oxidase from Rhodococcus erythropolis NCIMB 11540, peroxidase, 4-aminoantipyrine, and N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt. The developed method (named as PuO-POD-4AA-TOPS method) was applicable to bacterial culture supernatants. Furthermore, putrescine concentrations determined by the developed method roughly corresponded to the concentrations determined by HPLC.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteus mirabilis
/
Putrescine
Language:
En
Journal:
Anal Biochem
Year:
2020
Document type:
Article