A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease.
J Infect Dis
; 222(1): 158-168, 2020 06 16.
Article
in En
| MEDLINE
| ID: mdl-32052021
ABSTRACT
BACKGROUND:
Kawasaki disease (KD) is the leading cause of childhood acquired heart disease in developed nations and can result in coronary artery aneurysms and death. Clinical and epidemiologic features implicate an infectious cause but specific antigenic targets of the disease are unknown. Peripheral blood plasmablasts are normally highly clonally diverse but the antibodies they encode are approximately 70% antigen-specific 1-2 weeks after infection.METHODS:
We isolated single peripheral blood plasmablasts from children with KD 1-3 weeks after onset and prepared 60 monoclonal antibodies (mAbs). We used the mAbs to identify their target antigens and assessed serologic response among KD patients and controls to specific antigen.RESULTS:
Thirty-two mAbs from 9 of 11 patients recognize antigen within intracytoplasmic inclusion bodies in ciliated bronchial epithelial cells of fatal cases. Five of these mAbs, from 3 patients with coronary aneurysms, recognize a specific peptide, which blocks binding to inclusion bodies. Sera from 5/8 KD patients dayâ ≥â 8 after illness onset, compared with 0/17 infant controls (Pâ <â .01), recognized the KD peptide antigen.CONCLUSIONS:
These results identify a protein epitope targeted by the antibody response to KD and provide a means to elucidate the pathogenesis of this important worldwide pediatric problem.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Cells
/
Antibodies, Monoclonal
/
Antibody Formation
/
Mucocutaneous Lymph Node Syndrome
/
Epitopes
Limits:
Female
/
Humans
/
Infant
/
Male
Country/Region as subject:
America do norte
Language:
En
Journal:
J Infect Dis
Year:
2020
Document type:
Article
Affiliation country:
United States