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A bioconjugated MoS2 based nanoplatform with increased binding efficiency to cancer cells.
Kálosi, Anna; Labudová, Martina; Annusová, Adriana; Benkovicová, Monika; Bodík, Michal; Kollár, Jozef; Kotlár, Mário; Kasak, Peter; Jergel, Matej; Pastoreková, Silvia; Siffalovic, Peter; Majkova, Eva.
Affiliation
  • Kálosi A; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk.
  • Labudová M; Department of Cancer Biology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
  • Annusová A; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk and Centre for Advanced Material Application, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia.
  • Benkovicová M; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk.
  • Bodík M; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk.
  • Kollár J; Department of Synthesis and Characterization of Polymers, Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 41 Bratislava, Slovakia.
  • Kotlár M; University Science Park Bratislava Centre, Slovak University of Technology, Vazovova 5, 812 43 Bratislava, Slovakia.
  • Kasak P; Center for Advanced Materials, Qatar University, P.O. Box 2713, Doha, Qatar.
  • Jergel M; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk.
  • Pastoreková S; Department of Cancer Biology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
  • Siffalovic P; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk and Centre for Advanced Material Application, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia.
  • Majkova E; Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia. anna.kalosi@savba.sk and Centre for Advanced Material Application, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia.
Biomater Sci ; 8(7): 1973-1980, 2020 Mar 31.
Article in En | MEDLINE | ID: mdl-32065173
ABSTRACT
We evaluate the application of surfactant-free liquid-phase exfoliated MoS2 nanosheets as a nanoplatform for a cancer detection and treatment system equipped with an antibody-antigen based recognition element. Employing antigen-antibody binding, we increased the probability of the endocytosis of MoS2 nanosheets into CAIX expressing cells by 30%. The nanosheets are functionalized with a specific antibody M75, which forms an antigen-antibody complex with CAIX. The bioconjugation of MoS2 nanosheets involves biocompatible components with low cytotoxicity, verified in the tested cell lines by fluorescence-based cell viability assay. The cellular internalization is quantified by flow cytometry, while the internalization is confirmed by label-free confocal Raman imaging. Raman measurements show increased lysosomal activity in the proximity of the internalized nanoplatforms.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Disulfides / Carbonic Anhydrase IX / Molybdenum / Antibodies, Monoclonal / Neoplasms Limits: Humans Language: En Journal: Biomater Sci Year: 2020 Document type: Article Affiliation country: Slovakia Country of publication: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Disulfides / Carbonic Anhydrase IX / Molybdenum / Antibodies, Monoclonal / Neoplasms Limits: Humans Language: En Journal: Biomater Sci Year: 2020 Document type: Article Affiliation country: Slovakia Country of publication: ENGLAND / ESCOCIA / GB / GREAT BRITAIN / INGLATERRA / REINO UNIDO / SCOTLAND / UK / UNITED KINGDOM