Receptor and signaling pathway involved in bovine lymphocyte activation by Atractylodis macrocephalae polysaccharides.
Carbohydr Polym
; 234: 115906, 2020 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-32070525
ABSTRACT
The present study was to investigate the molecular mechanism underlying lymphocyte activation by total polysaccharides from Atractylodis macrocephalae (RAMPtp). The results showed that RAMPtp significantly promoted the secretions of cytokines (IFN-γ, IL-1α, IL-21, IFN-α, CCL4, CXCL9 and CXCL10), increased the proportions of CD4+ and CD8+ subpopulations, and enhanced the expressions of c-JUN, NFAT4, STAT1 and STAT3. microRNA sequencing identified 67 differentially expressed miRNAs (DEMs) in RAMPtp-stimulated SMLN lymphocytes, including 55 up-regulated and 12 down-regulated. GO and KEGG enrichment analyses of the predicted DEMs-targeted genes indicated that they were associated with immune system pathways, including PI3K-Akt, MAPKs, Jak-STAT and Calcium signaling pathways, which were confirmed by western blot and pathway inhibition assays. RAMPtp was further observed to favor immunostimulatory effect on both T and B lymphocytes via binding to TCR and membrane Ig individually. These ï¬ndings might explain the immunomodulatory mechanism of RAMPtp in ameliorating the bovine intramammary infection.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polysaccharides
/
B-Lymphocytes
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T-Lymphocytes
/
Atractylodes
/
Toll-Like Receptor 4
Limits:
Animals
Language:
En
Journal:
Carbohydr Polym
Year:
2020
Document type:
Article