Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans.

Lai, Wei-Chung; Sun, H Sunny; Shieh, Jia-Ching

Lai, Wei-Chung; Sun, H Sunny; Shieh, Jia-Ching.

Affiliation

Lai WC; Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan, ROC.
Sun HS; Institute of Molecular Medicine, National Cheng Kung University Medical College, Tainan, Taiwan, R. O. C.
Shieh JC; Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R. O. C.

Sci Rep ; 10(1): 2936, 2020 02 19.

Article in En | MEDLINE | ID: mdl-32076074

Subject(s)

Biological Assay/methods; Candida albicans/metabolism; Fungal Proteins/metabolism; Protein Interaction Mapping; Tetracycline/pharmacology; Candida albicans/drug effects; Candida albicans/genetics; Doxycycline/pharmacology; Fluorescence; Gene Expression Regulation, Fungal/drug effects; Genetic Markers; Hygromycin B/pharmacology; Luminescent Proteins/metabolism; Protein Binding/drug effects; Septins/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tetracycline / Biological Assay / Candida albicans / Fungal Proteins / Protein Interaction Mapping Language: En Journal: Sci Rep Year: 2020 Document type: Article Country of publication: United kingdom

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