Optimized CRISPR/Cas9 strategy for homology-directed multiple targeted integration of transgenes in CHO cells.

Shin, Sung Wook; Lee, Jae Seong

Shin, Sung Wook; Lee, Jae Seong.

Affiliation

Shin SW; Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea.
Lee JS; Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea.

Biotechnol Bioeng ; 117(6): 1895-1903, 2020 06.

Article in En | MEDLINE | ID: mdl-32086804

Subject(s)

CRISPR-Cas Systems; Gene Knock-In Techniques/methods; Transgenes; Animals; CHO Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Cricetulus; Plasmids/genetics; RNA, Guide, Kinetoplastida/genetics

Key words

CRISPR/Cas9; Chinese hamster ovary (CHO); double cut donor; homology-directed repair; multiple knock-in; targeted integration

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transgenes / Gene Knock-In Techniques / CRISPR-Cas Systems Limits: Animals Language: En Journal: Biotechnol Bioeng Year: 2020 Document type: Article Country of publication: United States

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