Whole-Genome Sequencing to Detect Numerous Campylobacter jejuni Outbreaks and Match Patient Isolates to Sources, Denmark, 2015-2017.
Emerg Infect Dis
; 26(3): 523-532, 2020 03.
Article
in En
| MEDLINE
| ID: mdl-32091364
ABSTRACT
In industrialized countries, the leading cause of bacterial gastroenteritis is Campylobacter jejuni. However, outbreaks are rarely reported, which may reflect limitations of surveillance, for which molecular typing is not routinely performed. To determine the frequency of genetic clusters among patients and to find links to concurrent isolates from poultry meat, broiler chickens, cattle, pigs, and dogs, we performed whole-genome sequencing on 1,509 C. jejuni isolates from 774 patients and 735 food or animal sources in Denmark during 2015-2017. We found numerous clusters; 366/774 (47.3%) clinical isolates formed 104 clusters of >2 isolates. A total of 41 patient clusters representing 199/366 (54%) patients matched a potential source, primarily domestic chickens/broilers. This study revealed serial outbreaks and numerous matches to concurrent food and animal isolates and highlighted the potential of whole-genome sequencing for improving routine surveillance of C. jejuni by enhancing outbreak detection, source tracing, and potentially prevention of human infections.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Campylobacter Infections
/
Disease Outbreaks
/
Campylobacter jejuni
/
Foodborne Diseases
/
Gastroenteritis
Type of study:
Etiology_studies
Limits:
Animals
/
Female
/
Humans
/
Male
Country/Region as subject:
Europa
Language:
En
Journal:
Emerg Infect Dis
Journal subject:
DOENCAS TRANSMISSIVEIS
Year:
2020
Document type:
Article
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA