Using zebrafish to assess the effect of chronic, early developmental exposure to environmentally relevant concentrations of 5-fluorouracil and leucovorin.
Environ Toxicol Pharmacol
; 76: 103356, 2020 May.
Article
in En
| MEDLINE
| ID: mdl-32120338
ABSTRACT
Environmental contaminants can deleteriously affect aquatic animals. One such contaminant is 5-fluorouracil (5-FU), a long-prescribed chemotherapeutic drug. Leucovorin (LV) is co-administered with 5-FU, potentiating its effects. Zebrafish (Danio rerio) larvae were reared in ng/L treatments of either 5-FU, LV, or a combined 5-FU/LV mixture for 8 dy. Survival was measured daily and swimming behavior assessed every other day. After 8 dy, larval length was measured, and densitometry of p53-labeled cryostat sections determined the extent of apoptosis. No significant differences in survival or apoptosis were found; larvae in the highest concentrations were largest. Changes in behavior of 5-FU-treated larvae were based on exposure duration; changes in LV-treated larvae were affected by drug concentration and duration. Larvae co-exposed to 5-FU/LV had responses like 5-FU-treated larvae. Overall, early developmental exposure of zebrafish larvae to environmentally-relevant concentrations of 5-FU and LV did not adversely affect survival, growth, and behavior suggesting realistic concentrations are sublethal and non-toxic.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Leucovorin
/
Environmental Pollutants
/
Fluorouracil
/
Antidotes
/
Antimetabolites
Limits:
Animals
Language:
En
Journal:
Environ Toxicol Pharmacol
Year:
2020
Document type:
Article
Affiliation country:
United States