Auto-fluorescent PAMAM-based dendritic molecules and their potential application in pharmaceutical sciences.
Int J Pharm
; 579: 119187, 2020 Apr 15.
Article
in En
| MEDLINE
| ID: mdl-32135228
ABSTRACT
The epithelial permeation of water-soluble fluorescent PAMAM dendrons based on 7H-benz[de] benzimidazo [2,1-a] isoquinoline-7-one as a fluorescent core across epithelial cell models MDCK I and MDCK II has been quantified. Hydrodynamic radii have been derived from self-diffusion coefficients obtained via pulsed-gradient spin-echo Nuclear Magnetic Resonance (PGSE-NMR). Results indicate that these dendritic molecules are molecularly disperse, non-aggregating, and only slightly larger than their parent homologues. MDCK I permeability studies across epithelial barriers show that these dendritic molecules are biocompatible with the chosen epithelial in-vitro model and can permeate across MDCK cell monolayers. Permeability is demonstrated to be a property of dendritic size and cell barrier restrictiveness indicating that paracellular mechanisms play the predominant role in the transport of these molecules.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Permeability
/
Epithelial Cells
/
Dendrimers
/
Fluorescent Dyes
Limits:
Animals
Language:
En
Journal:
Int J Pharm
Year:
2020
Document type:
Article